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Scientist, Reprogramming and Genome Engineering

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Positions
1
Close Date
15 Aug 2017
Location
CCRM

About CCRM:

CCRM, based in Toronto, Canada, is a unique not-for-profit group that is developing and commercializing cell and gene therapies and regenerative medicine technologies. We are leveraging our network of academics, industry partners and investors to tackle significant problems and advance our most promising technologies to the market to meet the needs of patients. For more information about CCRM, please visit our website at http://www.ccrm.ca.

 

Role Summary:

We are seeking a talented molecular and cell biologist to join our dynamic team. You must have a strong background in biochemistry with demonstrated ability in mammalian cell culture, molecular biology and cloning, working with DNA and RNA, performing polymerase chain reactions (PCR), and protein knockdown and protein analysis using western blots and immunocytochemistry. You will be a key member of the gene editing and reprogramming team and will be involved in assisting and directing projects to reprogram fibroblasts and blood samples to induced pluripotent stem cells (iPSCs). You will be involved in performing genome editing of these samples, and delivering the completed and characterized cell lines to clients. This work will require you to write project plans, develop assays, independently execute experimental plans, manage technical staff, analyze data, prepare data presentations and write thorough reports. 

 

Responsibilities:

  • Responsible for producing human iPSC from donor fibroblasts and blood samples.
  • Responsible for genome editing of human pluripotent stem cells (hPSCs), including gene correction and construction of reporter cell lines.
  • Planning and execution of experimental programs.
  • Experimental troubleshooting.
  • Supervision of technical staff.
  • Working effectively with other team members in a multi-disciplinary environment.
  • Writing and revising technical documents including standard operating procedures (SOPs), project plans and reports.
  • Developing and improving the skills and knowledge necessary to successfully support assigned projects.
  • Assisting and training others as necessary.
  • Documenting your work and preparing written and oral reports with accuracy and reliability.
  • Demonstration of integrity, credibility and a positive attitude in daily performance, and motivate others to exhibit these qualities as well.
  • Your responsibilities and role may change with the evolving needs of the organization, requiring flexibility and adaptability. 

 

Qualifications:

  • PhD in molecular or cell biology, or similar (two or more years relevant post-doctoral experience preferred).
  • Experience in conceiving, developing and independently carrying out a wide range of experiments and troubleshooting complex technical issues.
  • Familiarity with CL1/CL2 lab procedures, aseptic technique, safety procedures, and equipment.
  • Competent in mammalian cell culture including passaging, isolation, expansion and cryopreservation. 
  • Proven expertise in molecular biology including PCR, quantitative PCR (qPCR), protein knockdown, western blotting, and cloning.
  • Experience working with mechanisms of DNA repair and the use of engineered nucleases for genome editing (TALEN, ZFN and/or CRISPR).
  • Proven expertise in reprogramming to iPSCs from donor samples, including peripheral blood and fibroblasts is an asset.
  • Hands-on experience with differentiation of hPSCs is an asset.
  • Experience with cell characterization techniques, including flow cytometry, microscopy, high content screening platforms and qPCR.
  • Understanding of genetics and experience with associated research software (e.g. Geneious, clone manager, Vector NTI, UCSC).
  • Experiments and troubleshooting complex technical issues.
  • Understanding of developmental biology and factors controlling hPSC fate is an asset.
  • Detail-oriented self-starter with excellent analytical skills and the ability to multitask and succeed in a team environment.
  • Excellent English written and oral communication with strong record-keeping and documentation skills.

 

CCRM is a developing organization and represents a fluid working environment. Flexibility and adaptability are essential, and duties will be influenced by the needs of the organization.

 

Applications are due by 6:59 PM EDT on the date indicated at the top of this job posting.

Applicants must be legally eligible to work in Canada.

 

CCRM is committed to diversity and encourages applications from all qualified candidates. We will provide any requested accommodation to candidates with disabilities throughout the recruitment process.